Rabbit anti-SCD1 Polyclonal Antibody

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Product Details FAQ Citations(4) Video Pictures Documents |Overview |Synonym ACOD_HUMAN; Acyl-CoA desaturase; Delta(9)-desaturase; Delta-9 desaturase; Delta-9-Desaturase; FADS5; Fatty acid desaturase; PRO0998; SCD 1; SCD; SCD1; Stearoyl-CoA Desaturase |Host Rabbit |Specificity SCD1 |Species Reactivity Human, Mouse, Rat |Predicted Reactivity Dog, Pig, Cow, Rabbit, Sheep |Applications IHC-P=1:100-500, ICC=1:100 |Immunogen KLH conjugated synthetic peptide derived from human SCD1:265-359/359 |Properties |Concentration 1mg/ml |Purification affinity purified by Protein A |Clonality Polyclonal Antibody |Isotype IgG |Storage Temp. Store at -20 ° C for one yearAvoid repeated freeze/that cycles |Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |Research areas Cancer, Cardiovascular immunology, Signal transduction, Synthesis and degradation, Lipoprotein metabolism |Target |Background Stearoyl-CoA desaturase (SCD) is a regulatory enzyme in lipogenesis. The SCD1 gene is tightly regulated by signals such as insulin, leptin, carbohydrate, fatty acids and temperature. The enzyme is constitutively expressed in adipose tissue and is induced in liver in response to a fat free, high carbohydrate diet. SCD1 expression is higher in female than male mouse. The repression of RNA levels and enzyme activity of hepatic SCD1 by Leptin has been shown to significantly reduce the triglyceride storage, VLDL production and therefore an increase in fatty acid oxidation. Inhibition of SCD1 could be of benefit for treatment of obesity, hepatic steatosis and other metabolic disorders. |Cellular localization Cytoplasm; Cell membrane; |UniProt O00767 | Sample:Lung (Rat) Lysate at 40 ugPrimary: Anti- SCD1 (abs124771) at 1/300 dilutionSecondary: HRP conjugated Goat-Anti-rabbit IgG at 1/5000 dilutionPredicted band size: 41 kDObserved band size: 55 kD| Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (SCD1) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining. | Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-SCD1 Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining |Tips:This product is for research use only. Not for use in diagnostic prodcedures.