HEK293 cells stably expressing the human CGRP receptor are preincubated with various concentrations of MK 3207 in the presence or absence of 50% human serum for 30 minutes at 37 °C. Isobutyl-methylxanthine (300 ¦ÌM) is added to the cells, and then they are incubated for 30 minutes at 37 °C followed by stimulation with 0.3 nM ¦Á-CGRP for 5 minutes at 37 °C. After agonist stimulation cells are washed with PBS and the intracellular cAMP concentration is measured using the cAMP SPA Biotrak direct screening assay. Dose-response curve is plotted, and IC50 value is determined.

June 21, 2017

prudect name : HEK293 cells stably expressing the human CGRP receptor are preincubated with various concentrations of MK 3207 in the presence or absence of 50% human serum for 30 minutes at 37 °C. Isobutyl-methylxanthine (300 ¦ÌM) is added to the cells, and then they are incubated for 30 minutes at 37 °C followed by stimulation with 0.3 nM ¦Á-CGRP for 5 minutes at 37 °C. After agonist stimulation cells are washed with PBS and the intracellular cAMP concentration is measured using the cAMP SPA Biotrak direct screening assay. Dose-response curve is plotted, and IC50 value is determined.
MK 3207

Synonyms: CAS NO: 957118-49-9 957116-20-0(HCL)Molecular Formula: C₃₁H₂₉F₂N₅O₃Molecular Weight: 557.59Purity: 98% minSolubility: In DMSOStorage: -20°C

web site: www.medchemexpress.com

References PubMed ID：:http://www.ncbi.nlm.nih.gov/pubmed/18525307