Prediction of probability of oncogenic pathway activation or chemotherapy resistance in individual samples We used publicly available and validated gene expression read outs of oncogenic pathway activation previously generated by experimentally controlled activation of these pathways in vitro

April 7, 2017

he N-terminal portion of NS3 and its protease-dead mutant activated the Notch1 IC induced transcription of the Hes-1 promoter. These data suggest that the protease activity of NS3 is not…

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However, mass spectrometry had not been performed to unequivocally determine the mass of the native or recombinant Ehrlichia TRPs and the exact nature of the posttranslational modifications and the glycan attachment sites

nd TcdB are not essential for the toxins biological function, albeit determining the potency of the toxin by their interactions with cell surface structures. Furthermore, we monitored huge variations in…

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By recombinant expression and purification of full length TcdA and truncated TcdA1874 we were able to compare the cytopathic impact of both TcdA forms

April 6, 2017

ta presented in panels A.: For near-UV CD analysis, the mean residue molar ellipticities at 280 nm were plotted as a function of temperature. Tm values of 32.5uC and 33uC…

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Likewise, when we looked at the sections by fluorescence microscopy we observed that the number of calcein-labelled surfaces, as well as the distance between calcein labelling fronts was dramatically reduced in Col blasts, which were isolated from calvariae and differentiated ex vivo

llagenase treatment. Dissociated cells were fixed and stained for DNA synthesis and nucleus following manufacturer’s protocol. Cells were counted in a fluorescence microscope. In order to determine whether exposure to…

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We previously established a protocol for the activin-induced development of definitive endoderm during mouse ES cell differentiation

April 5, 2017

nsin mRNA expression: involvement of p38 MAPK, JNK, MKP-1, and NF-kappaB transcriptional factor in A549 cells. FEMS Immunol Med Microbiol 51: 17184. 57. Ye J, Ding M, Zhang X, Rojanasakul…

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we questioned whether the plant genes that are employed in response to Salmonella infection are different from those of animals Results Proliferation in plants does not alter the virulence for mammalian cells We examined the virulence potential of Salmonella enterica subsp

of full length and CROP-deleted toxins A and B towards polarized cells depend on the site of application To further investigate an alternative internalization mechanism for CROP- truncated C. difficile…

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TNFa has been proposed as a link between metabolic dysregulation and inflammation and/or vascular dysfunction in diabetes, and it is well established as a key molecule in diabetic retinopathy

April 1, 2017

1 | Volume 6 | Issue 2 | e14672 Estrogen-Dependent Genes Bioinformatic analysis and downstream annotation E-dependent DEG were ranked according to the methods described in the results section. Specific…

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Archive for April 2017